Clostridium difficile - the exotoxin produces the cellular injury. The responsible microorganisms are typically not seen in tissue section. Diagnosis is made by the detection of toxin.
2. Explain how you were able to identify the organism.
Based on the images provided, pseudomembrane plaques were observed. Also, she required multiple antibiotics which indicates that her normal intestinal flora was destroyed.
3. Which of the following are appropriate specimen samples for anaerobe culturing:
- blood, spinal fluid, abscess aspirate
- deep tissue biopsy, sputum, blood
- cerebrospinal fluid, tissue and debridement from decubitus ulcer, bile
- tissue swabs, blood, urine
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